ICMB Storeroom - MBB 1.426K (separate room for turning in invoice and for picking up items)
Can only pick up items from 9-12 AM and 2-5 PM
The Plan
We are going to work on our targets that we started picking in the Target Discovery Exercise. First, we will review those and see if they are good. Order overlap primers for our targets (we need these before we can start cloning for 'real') While we wait on the primers, we will express, purify and characterize YopH & FtHap enzymes - 2 current VDS targets. -- may also do NDM-1 and RpFabG
Then we will do some practice cloning procedures to teach you guys the new DNA cloning skills. Then we will proceed to work on cloning our targets Then we will express, purify and characterize our targets. Then we will perform enzyme activity assays to see if our targets work Then we will carry out inhibition assays
Wikispaces no like it when you have more than 3 windows open at a time! It be slow. Store reagents at the proper temperature & in the right location (e.g. correct freezer box) Label all reagents (tubes, boxes, liguids, powders) with VDS and Date (also include your initials if it is yours) Write down everything that you do in lab into your lab notebook Clean your area when done and return reagents and materials to their 'homes'
What are we doing today?
see the Daily Schedule Summ15==> Edit your Summer Student Schedules
Summer Researchers:
Mentors:Andee F, Charina B, Nikki W.
Alberto
Super Researcher: Anita V., Tony G.
Schedules
please enter your summer class schedule here (if you are also taking classes in the summer or have other conflicts with lab work):(Link TBD)
Mentor Schedules
Want to know when the mentors will be on? - check here (Link TBD)Start Date: Friday June 5th -----------------------------------> End Date: ~ July 31st
Group Meeting/Journal Club
once a weekLocation: GEA 100
Journal Club Wikispace Page
Group Meeting Slides are in the Google Drive folder
Spaces
PAI 2.14 wet labGEA 100
PAI 3.04H Dr. B's office
WEL 2.128 computer lab, WEL 2.144 computer lab
Painter 1st floor - ice room, autoclave, big centrifuge
Biotech lab - WEL 3.270
Robertus lab - WEL 5th floor
Chemistry Storeroom - WEL 1st floor (basement) - supplies and dry ice
Chemistry Electronics Shop - WEL 2nd floor (near computer lab)
BioScience Storeroom - NHB 1.502 - supplies
- NHB glassware shop - next to BioSci storeroo
ICMB Core Facilities:The Plan
We are going to work on our targets that we started picking in the Target Discovery Exercise.First, we will review those and see if they are good.
Order overlap primers for our targets (we need these before we can start cloning for 'real')
While we wait on the primers, we will express, purify and characterize YopH & FtHap enzymes - 2 current VDS targets.
-- may also do NDM-1 and RpFabG
Then we will do some practice cloning procedures to teach you guys the new DNA cloning skills.
Then we will proceed to work on cloning our targets
Then we will express, purify and characterize our targets.
Then we will perform enzyme activity assays to see if our targets work
Then we will carry out inhibition assays
Lab Safety:
Have you complete your lab safety trainings?Tours and Fun Stuff
TACC Tour at VisLab in ACES building ON CAMPUS - @
TACC Machine room Tour - @
GASF (Genome Sequencing and Analysis Facility) Tour in MBB 4.102 (not done)
Skills List:
Target DiscoveryPCR (primer overlap)
Restriction Enzyme Digest
Cloning
Plasmid Midi-Prep
DNA Sequencing
Protein Expression, Purification, Characterization (Large scale)
FPLC
Enzyme assays
Things to Keep in Mind
Wikispaces no like it when you have more than 3 windows open at a time! It be slow.Store reagents at the proper temperature & in the right location (e.g. correct freezer box)
Label all reagents (tubes, boxes, liguids, powders) with VDS and Date (also include your initials if it is yours)
Write down everything that you do in lab into your lab notebook
Clean your area when done and return reagents and materials to their 'homes'